An Alternative to Increase Accumulation of Phenolic Compounds in Grapevine Callus Cultures: Chemical Mutagen Applications

This research was carried out to investigate the effects of chemical mutagens on producing phenolic compounds in calli obtained from Royal grape variety. Petioles were cultured B5 medium (0.5 mg/L benzyl amino purine and 0.5 indole acetic acid) obtain callus. Calli subcultured same nutrient three times at one month intervals. In fourth subculture, transferred media with composition containing ethyl methanesulfonate, sodium azide, azacitidine acridine orange different doses mM, 1.0 2.0 mM) these mediums for 60, 120 180 minutes then they four weeks mutagen-free content as their previous mediums. After that total compound, flavanol, flavonol anthocyanin analyzes performed. As a result, all applied are effective increasing production depending dose time. Keywords: Grapevine, Chemical mutagen, Phenolic compounds, Anthocyanin. DOI: 10.7176/JBAH/11-22-05 Publication date: November 30 th 2021